Detection of terminal mismatches on DNA duplexes in homogeneous assays or with immobilized probes.
نویسندگان
چکیده
We recently reported the design of new fluorescent oligo-2'-deoxyribonucleotides (FODNs) for the detection of terminal mismatches on DNA duplexes in homogeneous assays. We now report the validation of this method in homogeneous assays with other sequences and the feasibility of the detection of terminal mismatches with immobilized FODNs. In all cases studied, the mismatched duplexes were more fluorescent than the perfect ones and results confirmed that the discrimination factor is sequence-dependent.
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ورودعنوان ژورنال:
- Nucleosides, nucleotides & nucleic acids
دوره 26 10-12 شماره
صفحات -
تاریخ انتشار 2007